HPLC determination of ursolic acid in Leshan Gardenia.docVIP

 PAGE \* MERGEFORMAT 11 HPLC determination of ursolic acid in Leshan Gardenia Author: Pu Qing-Rong, TANG Can, Zhang Yanyan, Yun-Peng Li, Zhao, Liu Zhi rights [Abstract] Objective To control the quality of Leshan gardenia, establish high-performance liquid chromatography Leshan Gardenia ursolic acid method. Methods using high performance liquid chromatography with ursolic acid as a chemical reference substance, stationary phase: UltimateTM C18 bonded silica gel column (4.6 mm * 250 mm, 5 μ m), mobile phase: methanol: water: acetic acid: triethylamine (90:10:0.03:0.06), flow rate 0.6 ml * min-1, temperature 25 ℃ , wavelength 210 nm measured Leshan Gardenia ursolic acid. The results of ursolic acid in the range of 0.1 ~ 3.0 μ g in peak area A with the quality of M showed a good linear relationship, the regression equation: A =- 12 954.882 9 +452 850.960 3 M, r = 0.999 6, n = 7; 3 groups Leshan Gardenia content of ursolic acid, respectively (0.255 5 ± 0.001 6) mg * g-1 medicines, RSD = 0.622 1%, n = 4; (0.265 825 ± 0.003 491) mg * g-1 medicines, RSD = 1.313%, n = 4; (0.258 875 ± 0.006 198) mg * g-1 medicines, RSD = 2.394%, n = 4; precision experimental RSD = 1.754%, good precision instruments; stability of the experimental RSD = 3.493 % in 3.5 h Determination of ursolic acid is stable; reproducible experimental RSD = 0.905 1%, average recovery was 101.9%. Conclusion Leshan Gardenia High Performance Liquid Chromatography for Determination of Ursolic Acid, this method is simple, stable, precision, reproducibility, recovery is better, worthy of promotion. [Keywords:] Leshan Gardenia; Ursolic acid; Determination; high performance liquid chromatography Abstract: ObjectiveTo establish the method of determining the cotent of ursolic acid in Fructus Gardeniae by HPLC. MethodsHPLC was used to determine the content of Ursolic acid in Fructus Gardenice ursolic acid as the standard, Solid phase: UltimateTM C18 (4.6 mm * 250 mm, 5 μ m), Liquid phase: Methyiaicohol: W