Human adenoid cystic carcinoma multidrug resistance cell line.docVIP

 PAGE \* MERGEFORMAT 23 Human adenoid cystic carcinoma multidrug resistance cell line Author: Xiao-Dong Zhou Song Guoxiang Sui Zhi Fang Ji-Wu Chang 【Abstract】 Objective: To establish a human adenoid cystic carcinoma of drug-resistant cell line, in order to clarify the adenoid cystic carcinoma of the reversal of multidrug resistance mechanisms and to provide models and research basis for drug resistance. Methods: vincristine (VCR) as the induction agent, by increasing the concentration of intermittent stimulation of human adenoid cystic carcinoma cell line (ACC) in vitro induced resistance to the establishment of resistance to VCR of adenoid cystic carcinoma cell line ACC / VCR. Cell count cell growth curve mapping, thiazole blue (MTT) colorimetric assay cells to chemotherapeutic drug sensitivity. Results: ACC in vitro after induction, ACC / VCR cells to vincristine (VCR), adriamycin (ADM) and pingyangmycin (PYM) markedly enhanced resistance, with cross-resistance of cyclophosphamide ( CTX), fluorouracil (5-FU) and cisplatin (DDP) resistance without significant changes. Resistance before and after the ACC cell growth curve, population doubling time and the light microscope, no significant changes in patterns. Conclusion: VCR adenoid cystic cancer cells can induce multi-drug resistance. Keywords: adenoid cystic carcinoma multidrug resistance of vincristine Establishment of multidrug cell line of human adenoid cystic carcinoma Abstract AIM: To establish an in vitro resistance model of adenoid cystic carcinoma which can provide theoretical information for elucidating the mechanisms of multidrug resistance (MDR), improving the chemotherapy scheme, evaluating the prognosis and reserving the MDR in adenoid cystic carcinoma.METHODS: The resistance cell line, ACC / VCR, was induced in the ACC cell line in vitro by progressive concentrations of vincristine (VCR), a drug of choice in the treatment of adenoid cystic carcinoma.