HPLC Determination Qushu content of glycyrrhizic acid capsule.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Determination Qushu content of glycyrrhizic acid capsule [Abstract] Objective To establish a Qu Shu capsule method for determination of glycyrrhizic acid. Methods HPLC method, Kromasil C18 column (250 mm * 4.6 mm, 5 μ m); mobile phase was methanol -0.2 mol / L ammonium acetate - acetic acid (67:33:1); flow rate of 0.8 mL / min; detection wavelength 250 nm. Results Qushu capsule determination of glycyrrhizic acid content in the linear range of 188.06 ~ 494.59 μ g / mL; the average recovery was 103.3%, RSD of 2.2% (n = 6), theoretical plate number is greater than 2000. Conclusion HPLC Determination of glycyrrhizic acid content Qushu capsule is simple, accurate and repeatable. [Keywords:] HPLC; Qushu capsule; glycyrrhizic acid; content Qu Shu Capsule mainly by patchouli, incense Ru, licorice and other composition, clinical use for many years, for the treatment of heat stroke caused by a sense of aversion to cold cold fever embolism. This paper selected the main drug Fang licorice contains glycyrrhizin as the major components of indicators, refer to the Chinese Pharmacopoeia [1], using high performance liquid chromatography assay method of Qu Shu Capsule study in order to control the quality of the preparation. An experimental drug, reagent Qu Shu Capsule (batch number 20,050,819), glycyrrhizic acid ammonium salt a single reference substance (China Pharmaceutical and Biological Products Institution, batch number 110731-200205), methanol (HPLC pure), water for ultra-pure water and the remaining reagents were analytical pure. 2 Experimental apparatus United States Varian Prostar 210-type high-performance liquid chromatograph, Varian Prostar 325 UV - visible detector. 3 Experimental methods and results 3.1 Chromatographic conditions Kromasil C18 column (250 mm * 4.6 mm, 5 μ m); mobile phase was methanol -0.2 mol / L ammonium acetate - acetic acid (67:33:1); flow rate of 0.8 mL / min; detection wavelength 250 nm. 3.2 Preparation