HPLC determination of Xiasangju Granules Linarin content.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination of Xiasangju Granules Linarin content Author: Huang Xiaowen, Xieyan Ting, Feng Jia Xin, Xie Feng Luan [Abstract] Objective To establish Xiasangju Granules Linarin determination method. Methods RP-HPLC, column: DiamonsilTMC18 column (250 mm * 4.6 mm, 5 μ m, Dumas companies); mobile phase was methanol   tetrahydrofuran-water (43:5:52), detection wavelength 334 nm The flow rate was 1.0 mL * min-1, column temperature 30 ℃ . Results Linarin 0.048 ~ 0.959 μ g in the context of linear relationship was good, r = 0.9999, average recovery was 100.7%, RSD of 0.86% (n = 6). Conclusion The established method is accurate, reproducible, high sensitivity, can be used for the preparation of quality control. [Keywords:] Xiasangju particle; Linarin; high-performance liquid chromatography Close Xiasangju particles contained in the ‘Ministry of Health, pharmaceutical standard’ medicine Narikata Standard No. 15, from Prunella vulgaris, wild chrysanthemum, mulberry leaves composed of three Chinese herbal medicines, with Liver eyesight, Shufeng cooling effect for the treatment of Fengreganmao, Mu Chi headache, dizziness, tinnitus, sore throat embolism. Chrysanthemum is a major component of this preparation, one is a wild chrysanthemum Linarin characteristic elements [1-2], literature [3-5] reported that high performance liquid chromatography preparation of wild chrysanthemum Linarin content, the main plus methanol using ultrasonic treatment or reflux extraction method, this paper, according Linarin of water-insoluble nature of the [6], in order to extract the n-butanol extraction method Linarin build high-performance liquid chromatography Xiasangju Granules Linarin content approach, accurate, sensitive, negative control without interference, for the preparation provide the basis for quality control. An instrument and reagent 1.1 Instrument Shimadzu High Performance Liquid Chromatography (LC  10Avp solvent delivery pump, SPD 