Human amniotic membrane transplantation in ophthalmology.docVIP

 PAGE \* MERGEFORMAT 17 Human amniotic membrane transplantation in ophthalmology [Keywords:] eye; amniotic membrane; tissue transplantation In 1995, Kim and Tseng [1] the use of 4 ℃ 100% glycerol preserved human amniotic membrane transplantation into severely damaged rabbit ocular surface, obtained the success of ocular surface reconstruction. Since then, the application of amniotic membrane transplantation in ophthalmology areas have been gradually widened. A membrane anatomy and histological features of Human amniotic membrane thickness of 0.02 ~ 0.5mm, transparent, toughness, by a thick basement membrane and non-vascular non-neural and lymphatic vessels of matrix composition. From the inside to outside is divided into five layers: epithelial cell layer, basement membrane, compact layer, fibroblast cell layer and sponge layers. In the basement membrane surface and there is a potential gap between the chorion and easy blunt separation. Mature amniotic cell surface and the side has many cylindrical shape arranged in regular or irregular microvilli, their participation in the formation of the complex distorted lost pipeline system between cells. In vitro experiments and tissue culture cells, epithelial cells showed synthesis, secretion and deposition of basement membrane, extracellular matrix components capacity. The basement membrane of amniotic membrane is the body of the most thick basement membrane, in order to 0.1μ m, containing collagen type Ⅳ collagen and other ingredients. The basement membrane under the dense layer thickness ranging from 5 ~ 50μ m, its main function is to support the growth of surface epithelium [1,2]. Therefore, amniotic cells lost between the pipeline system was conducive to material exchange; abundant collagen enhances its toughness; the same time, transparent, non-vascular and other organizations to become more desirable features to make the eyes of amniotic membrane surface reconstruction for the body material. Two amnioti