HPLC fingerprint of impatient.docVIP

 PAGE \* MERGEFORMAT 6 HPLC fingerprint of impatient [Abstract] Objective To establish the fingerprint impatient, impatient full control of quality medicines. Methods HPLC impatient to establish eight herbs in different parts of the fingerprint. Results in the selected chromatographic conditions, the calibration of the 13 peaks. Conclusion The method is stable, reliable, and can be used for impatient Fingerprinting. [Keywords:] impatient, fingerprints, HPLC Impatient as Impatiens Impatiens Impatiens balsamina L. plants dry mature seeds, summer and autumn harvest when the seed is about to mature. Impatient with Ruanjian product, lower gas line addiction, Speranskia the role of blood circulation through gluten [1]. Impatient source of rich, cultivated all over the country, in order to examine differences in their chemical composition, we established 8 groups impatient fingerprint of medicinal herbs. fingerprinting as a multi-component complex samples of the effective mass method can reflect the sample integrity, characteristic, through impatient fingerprints herbs, medicinal herbs for the establishment of a comprehensive and effective quality control method for the identification of different areas and applications impatient to provide technical support. 1 equipment, reagents and medicine U.S. Agillent 1100 high performance liquid chromatography, two array detector. Acetonitrile was HPLC grade water as Wahaha purified water .8 batches of samples were Shandong, Henan, Xian, Hubei, Anhui, Shenyang, Sichuan, Beijing, medicine, herbal Table 1. Table 1 impatient herbal (slightly 2 Methods 2.1 Chromatographic conditions for the Dumas company column C18 (4.6 mm 200 mm, mobile phase: 0.1% formic acid (A-acetonitrile (B, gradient elution procedures (B, 0 ~ 15 min, 25% ~ 90%, 15 ~ 20 min, 90%, 20.01 ~ 25 min, 25%. volumetric flow rate of 1.0 ml / min, column temperature was 30 , the detection wavelength was 260 nm. 2.2 Preparation of test solutio