HPLC method for simultaneous determination of water-soluble medicine Danshen active ingredient content of.docVIP

 PAGE \* MERGEFORMAT 9 HPLC method for simultaneous determination of water-soluble medicine Danshen active ingredient content of Authors: Yang Sheng, Song smooth, Zhao Jianbang, DING [Abstract] Objective To establish a HPLC method for simultaneous determination of Salvia Salvia Salvia and Gansu sodium, protocatechuic aldehyde and Sal B-content, through three kinds of water-soluble active ingredient measurement, evaluation of wild and cultivated Salvia Gansu, wild and cultivated Gansu Salvia quality. Methods The analytical column was C18 column (4.6 mm * 250 mm, 5 μ m); mobile phase was methanol - acetic acid - water (20:80:1); flow rate of 1.0 mL / min. Binary gradient elution. Results Danshensu sodium, protocatechuic aldehyde, Sal B at 58 ~ 1455 ng, 15 ~ 162 ng, 825 ~ 8250 ng range a good linear relationship, recoveries were 102.20%, 101.53% and 103.03%. Conclusion The method is simple, accurate and good separation effect can be used as three kinds of Salvia miltiorrhiza water-soluble active ingredients, while quantitative determination. [Keywords:] high-performance liquid chromatography; Salvia; Gansu Salvia; water-soluble components; Salvia sodium; protocatechuic aldehyde; Sal B; cultivation; Wild Salvia ‘Chinese Pharmacopoeia’ income set for the Labiatae plant Salvia Salvia miltiorrhiza Bge root [1], located in Gansu, Tianshui and Longnan region, the only resources, but not commodities, in recent years, Qingyang, Pingliang and so the success of artificial cultivation. Gansu Salvia plants from Labiatae Salvia przewalskii Salvia przewalskii Maxim. Or a variant of brown hair Salvia przewalskii Salvia przewalskii var. Mandarinonum (Diels) Stib.’s Dried root and rhizome, located in Gansu, Sichuan and Yunnan provinces area, a place for traditional Chinese medicine Danshen habit supplies, medical history, longer in Gansu, Qing gazetteers have been included [2], is also scheduled for the West, Pingliang and so successfully planted. Artificial cultivation of Sal