Human bone marrow mesenchymal stem cells on allogeneic regulatory T cells and the possible mechanism of experimental study.docVIP

 PAGE \* MERGEFORMAT 2 Human bone marrow mesenchymal stem cells on allogeneic regulatory T cells and the possible mechanism of experimental study Authors: Yang Jing, Qing-Hai Wang, Qiu-Tang Zeng, Xiao-Bo Mao [Abstract] This study investigated human bone marrow-derived mesenchymal stem cells (human mesenchymal stem cells, hMSC) allogeneic CD4 CD25 regulate T cells, and further identification of human bone marrow-derived mesenchymal stem cells on T-lymphocyte immune regulation may mechanism. Isolated and cultured from human bone marrow hMSC, was detected by immunohistochemistry and characterized the surface markers. From the unrelated donor peripheral blood of healthy individuals extracted mononuclear cells under the action of IL  2 in vitro, the experimental group with different order of magnitude hMSC (1 * 103,1 * 104,1 * 105) were cultured for 5 days, the control group join PBS. At the end of the train before the 18 hours after 3H  TdR labeled by β liquid scintillation counting T lymphocyte proliferation detected by flow cytometry in each group the percentage of CD4 CD25 T cells, RT  PCR detected cells in each group the relative expression levels of Foxp3 (Foxp3 / β  actin), ELISA in the supernatant were measured TGF  β , IL  10, IFN  γ and IL  12 expression. Pearson examination and analysis of the relative expression of Foxp3 and CD4 CD25 T cells in the relevant percentage, as well as Foxp3 relative expression, CD4 CD25 T-cell percentage and the degree of T cell proliferation (counts per minute, CPM value), cytokine TGF  β , IL  10, IFN  γ and IL  12 expression correlation. The results showed that: hMSC could inhibit T lymphocyte proliferation, and inhibition in a dose-dependent manner with the hMSC; experimental group in each group CD4 CD25 T cell subsets in content and relative expression level of Foxp3 compared with control group significantly increased (p