Increase of low-dose cisplatin in human cervical carcinoma Hela cells and Siha light sensitivity of photodynamic therapy.docVIP

 PAGE \* MERGEFORMAT 17 Increase of low-dose cisplatin in human cervical carcinoma Hela cells and Siha light sensitivity of photodynamic therapy Of: Wang Haiyan, ZHANG You-zhong, JIANG Kan, Jiao Hui, Zhao-Jing [Abstract] Objective To investigate the increase of low-dose cisplatin in human cervical carcinoma cell lines Hela and Siha photodynamic therapy light sensitivity and the mechanism of apoptosis. Methods and Siha Hela cells were divided into control group, photodynamic therapy group, cisplatin alone group and photodynamic therapy plus cisplatin treatment group, MTT assay of each group of human cervical cancer cell lines Hela and the proliferation of Siha. immunocytochemical method to detect the effect of Hela and Siha cell receptor protein after P185c erbB 2 expression. Results of photodynamic therapy of cisplatin and the combination led to the extent of apoptosis was significantly stronger than that of single cisplatin or photodynamic therapy, cells P185c erbB 2 protein levels also decreased in line with them. But before cisplatin Photodynamic therapy and after the first photodynamic therapy with cisplatin compared to effect not significantly different. Conclusions of cisplatin and photodynamic combination therapy in vitro and in human cervical cancer Hela cells significantly inhibited the proliferation Siha, stronger than a single method treatment, the mechanism may be related to the inhibition P185c erbB 2 expression. [Keywords:] 5 ALA; cisplatin; photodynamic therapy; immunocytochemistry; cervical cancer cell lines ABSTRACT: Objective To investigate the mechanism of small dose cisplatin in enhancing photodynamic therapy sensitivity and apoptosis in human cervical carcinoma cell lines.Methods The Hela and Siha cells were divided into four groups (blank control group, photodynamic therapy only, cisplatin only, photodynamic therapy and cisplatin). The effects on proliferation of Hela and Siha cells in the four groups were examined by MTT assay