Indirect method and the specificity of IgM capture assay sensitivity and specificity compared.docVIP

 PAGE \* MERGEFORMAT 14 Indirect method and the specificity of IgM capture assay sensitivity and specificity compared Of: Marxism-Leninism-ting, Wang Yawen, Yang Duo, Yuan Yukang [Abstract] Objective To compare the capture IgM and indirect detection specificity and sensitivity when the differences and to explore the causes of the differences. Methods Using indirect ELISA, capture ELISA assay and active hepatitis B and renal syndrome heat (HFRS) IgM levels in patients with samples and compared between the detection rate, detection threshold, specific differences. diluted in PBS in the results of testing anti-mouse IgM antibodies, the specificity and the two methods similar sensitivity to detect active hepatitis B and HFRS patient specimens, the sensitivity of indirect detection of IgM capture method was significantly lower than that detected by the second-mercaptoethanol treatment group and the HFRS group were hepatitis B, two were negative, indicating that Both methods have IgM antibodies against type-specific, cross-experimental results show that detection of IgM, the two reagents with specificity for antigen. Conclusion The early diagnosis of acute infection, latent virus infection and chronic infection should be re-activated capture assay with IgM, IgM indirect assay for the diagnosis of acute infectious diseases need to be further explored. [Keywords:] Hepatitis B HFRS indirect ELISA IgM capture ELISA ABSTRACT: Objective To assess the differences in the specificity and the sensitivity in detecting specific IgM by indirect ELISA and capturing-ELISA methods, and to analyze the possible causes of those differences. Methods The HBc-IgM and EHF-IgM level in serum samples from activity Hepatitis B patients and hemorrhagic fever patients with renal syndrome were determined by both indirect-ELISA and capturing-ELISA methods, and the differences in the positive rate, positive threshold value and the specificity between those two methods were compared. Re