Infrasound in male mice testicular cells DNA CpG methylation levels in.docVIP

 PAGE \* MERGEFORMAT 6 Infrasound in male mice testicular cells DNA CpG methylation levels in Author: Gao Liu Village, Tsai-Yun Chen, Zhou Peng, CHEN Jing algae, Shuqing [Keywords:] infrasound; mouse; testicular cells; methylation [Abstract] AIM: To investigate the changes of genome DNA methylation of testicular cells in mice with high performance liquid chromatography (HPLC) after infrasound exposure, and to explore the relations between infrasound and epigenetics. METHODS: The BALB / c mice were exposed to infrasound (8 Hz/130 dB) for 1, 7 and 14 d, respectively, 2 h daily, and the control groups were unexposed. Then the changes of genome DNA methylation of testicular cells in the mice were analyzed with HPLC. RESULTS: The levels of the genome DNA methylation of testicular cell in exposed groups were all significantly lower than those in control groups as time went on (1 d, 0.0150 ± 0.0127 vs 0.0235 ± 0.0062; 7 d, 0.0140 ± 0.0030 vs 0.0273 ± 0.0040; 14 d , 0.0150 ± 0.0096 vs 0.0250 ± 0.0049. P = 0.000). CONCLUSION: The infrasound markedly affects the genome DNA methylation of testicular cells. These changes are positively associated with the infrasound exposure time. [Keywords] infrasound; mice; testis cell; methylation [Abstract] Objective: The high-performance liquid chromatography analysis of the role of infrasound testicular cells in mice genomic DNA CpG methylation analysis of the changes in order to study the role of infrasound and the relationship between epigenetic changes. Methods: 8 Hz , 130 dB infrasound were acting on the 1, 7 and 14 d group of three experimental groups, each male Balb / c mice 20, a day infrasound exposure 2 h. At the same time as control group, and the use of high-performance liquid chromatography analysis of the experimental group and control group mouse testis genomic DNA CpG methylation level were detected. Results: infrasound exposure to 1, 7 and 14 d group of cells in mouse testis genomic DNA CpG methylation leve