Inhibition of formaldehyde on fungi isolated.docVIP

 PAGE \* MERGEFORMAT 5 Inhibition of formaldehyde on fungi isolated [Keywords:] fungi formaldehyde anatomical specimens , The Western education attach importance to human tissue for morphological studies, the body of currently used anti-corrosion specimens were fixed after treatment in order to avoid water evaporation, rotten and other changes, often soaked in formalin (40% formaldehyde) conducted in the wet to save [ 1]. In order to anatomy teaching experiments in cadavers to set a suitable concentration of formaldehyde, the author has conducted some experiments. 1 Materials and methods 1.1 Materials by formaldehyde perfusion better placed in clean water to save the body of a period of time scraped the surface of a pure white velvet-like fungi (gross bacteria), and brown villous fungus (Aspergillus) two kinds. Different concentrations of formaldehyde: 15%; 20%; 25%; 30%; 35%; 40%; 45%. 1.2 Methods from the scraped the surface of the body of pure white cashmere-like fungi (gross bacteria), and brown villous fungus (Aspergillus) This two kinds of fungi derived after the put in a weak sterile Sabouraud’s plate medium, crossed vaccination completed placed in 28 ℃ incubator mold incubated 7 d, when the entire mold is covered with flat medium surface, there are granular, after growth of spores and fungal hyphae, as the bacteria to use it. To 1 mL under test drugs (the above-mentioned seven kinds of different concentrations of formaldehyde solution) into sterile 9 mm diameter glass petri dish, and then poured into 56 ℃ to them about the weak Sabouraud’s medium 20 mL, the full mixing of drugs later, still place a moment, to be solidified agar medium. After production of the medium, each medium whichever is three, repeat the test, respectively, to different concentrations of the drug culture medium inoculated completed the above-mentioned two kinds of cultured bacteria that grow well in its surface, mold set 28 ℃ incubator incubated 7 d, observe the results. 2