JIEAN-PEPTIDE Mechanism of anti-fungal.docVIP

 PAGE \* MERGEFORMAT 19 JIEAN-PEPTIDE Mechanism of anti-fungal [Abstract] Objective To study the Czech Republic, an antifungal peptide hormone mechanism. Methods The morphological method and isotope labeling. Morphology observed by joanne peptide hormone treatment, the morphological changes of the tested fungi. Further use of the specific 14C isotope-labeled substrate ‘uridine diphosphate - (14C) - glucose’ tracer to study the AGN peptide hormone pairs of fungi (1,3)-β -D-glucan synthase activity Reaction . The results show that microscopic morphology by joanne peptide hormone treatment, for the test fungi showed spherical expansion, and with the jet on the role of peptide hormone intensity increased, spherical expansion of the extent and frequency also increased, suggesting that jet on the role of peptide hormone loci in the fungal cell wall. Isotope labeling experiments show that AGN peptide hormone can suppress (1,3)-β -D-glucan synthase activity, reducing fungal cell wall synthesis process (1,3)-β -D-glucan radioactivity incorporation of Phytophthora and Colletotrichum capsici (1,3)-β -D-glucan synthase inhibition rates were 77.7% and 77.5%. Conclusion JIEAN-PEPTIDE anti-fungal mechanism as follows: suppressor cells (1,3)-β -D-glucan synthase activity, destruction of fungal cell wall synthesis, resulting in fungal cell wall component (1,3)-β -D-glucan defects and pathogenic fungi growth inhibition. [Keywords:] joanne peptide hormone; anti-fungal mechanism; (1,3)-β -D-glucan synthase A Research on Mechanism of Antifungal Polypeptide Jiean-peptide DAI Fu-ying1, ZHOU Jin-yan2, TAN Hong2 (1.Department of Pathogenic Biology, Chengdu Medical College, Chengdu 610083; 2.Chengdu Institute of Biology, CAS, Chengdu 610041) Abstract: Objective To investigate the antifungal mechanism of Jiean-peptide.Methods The mode of action of Jiean-peptide against 4 phytopathogenic filamentous fungi associated with phytopathy: Phytophthora sp., Colletotrichum gossypii, Sout