Jinhong Tablet on the impact of cholangitis in rat thymus.docVIP

 PAGE \* MERGEFORMAT 8 Jinhong Tablet on the impact of cholangitis in rat thymus Author: Zhang Jing Zhang Xue-Lin Gao Ju Ping Shen Pei-Ting Zhu [Abstract] Acute cholangitis central state of cell-mediated immunity changes in the thymus, as well as traditional Chinese medicine Jinhong tablets intervention role. Methods: Male SD rats of clean grade 24, were randomly divided into three groups of eight. Common bile duct ligation alone group (referred to as simple ligation group): bile duct ligation, but not injection of bacteria, post-operative feeding saline; model group: the establishment of acute cholangitis model was fed with normal saline after surgery; Jin-hong Tablets Group: modeling post-operative feeding Jinhong film suspension. The rats were killed 5 d after surgery, thymus derived, making the thymus electron microscopy and light microscopy observation, and testing the quality of the thymus, thymus index, apoptotic index, thymus protein encoded by Bcl-2 gene expression and thymus expression of Fas gene encodes proteins. Results: The frequency of morphologically apoptotic cells ranked as the model group, Jinhong in treatment group and the simple ligation group. Model group, the thymus and thymus index were lower quality, and Jinhong in treatment group and compared with simple ligation group, the difference was statistically significant. Thymus apoptosis index in model group was significantly higher than in treatment group and Jinhong simple ligation group. Jin Hong-chip treatment group, Bcl-2 gene encodes protein expression is higher than the model group, Fas gene encodes protein expression was no significant difference between the two groups. Conclusion: Acute cholangitis in the quality of rat thymus and thymus index decreased thymus apoptosis index increased, non-physiological apoptosis. Antipyretic and purgative herbs Jinhong films have a certain control this kind of non-physiological apoptosis, this effect may be inhibiting apoptosis by up-regulating