Jiuxie Granules lesions of rats with ulcerative colitis of oxidative damage_0.docVIP

 PAGE \* MERGEFORMAT 12 Jiuxie Granules lesions of rats with ulcerative colitis of oxidative damage Of: Li Hailong, Wu Yuhong, Chu Huiyuan, car Min, Anfang Yu, Hongwei [Abstract] Objective To observe Jiuxie Granules rat model of ulcerative colitis, and to explore its mechanism. Methods heterogeneous sensitized allogeneic colonic enema method and the acetic acid method of combining the local manufacture of animal models. The Wistar rats were randomly divided into control group, model group, positive control group and Jiuxie Granules large, medium and low-dose group. continuous administration of 14 d, 24 h after the last administration rats were killed to prepare the colon tissue, biochemical detection of colon tissue glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), total nitric oxide synthase (tNOS ) and inducible nitric oxide synthase (iNOS) levels. Results Jiuxie Granules can significantly reduce colon tissue NO, tNOS, iNOS and MDA in the activity or level, to improve GSH-Px, SOD activity. Conclusions Chronic Diarrhea Granules for ulcerative colitis rats a significant intervention effect, which may be free radical damage and antioxidant, inhibit NO, tNOS and iNOS production, increased antioxidant enzyme activity. [Keywords:] Jiuxie Granules, ulcerative colitis, glutathione peroxidase, malondialdehyde, superoxide dismutase, nitric oxide, nitric oxide synthase, rat Abstract: Objective To observe the effects of Jiuxieling granules on rats with ulcerative colitis and explore its mechanism. Methods Ulcerative colitis models were established by immune method combined with local stimulation of 5% acetic acid. Wistar rats were randomly divided into control, model, positive control and low, middle, high dose Jiuxieling granules group. Rats in positive control group were treated with sulfasalazine, and those in three treatment groups were treated with low, middle and high dosage of Jiuxieling granules res