Ketamine-induced changes in hypothalamic gene expression profiles.docVIP

 PAGE \* MERGEFORMAT 13 Ketamine-induced changes in hypothalamic gene expression profiles Author: Jia-Hai Ma, Xu Li-xian, Wang Huixia, Zhang Hui, Zhou Lihua [Keywords:] Ketamine;, gene chip;, gene expression profile;, thalamus;, rat Alteration of gene expression profile in rat thalamus induced by ketamine [Abstract] AIM: To have more insight into the alteration of gene expression profile in rat thalamus anesthetized with ketamine using gene chip. METHODS: Four SD rats were randomly divided into 2 groups: control group administered intraperitoneally with 0.9% saline, and ketamine group with 100 mg / kg ketamine. One hour later, thalamus was dissected and the total RNA was isolated. cDNA was amplified through reverse transcription reaction. Biotinlabeled cRNA was synthesized using cDNA as template. After purification and fragmentation, the cDNA was hybridized with rat neurobiology U34 gene chips. Chips were scanned and genes were analyzed using Microarray Suite Version 5.0 software. Differentially expressed genes were classified according to their biological function. RESULTS: Among the total 1323 probes detected, 237 genes showed differential expression in ketamine group. The expression level of 132 genes increased and 105 genes decreased, and 59 genes among them had the expression level alteration over 2 times. The differentially expressed genes were mainly classified into ion channels, signal transduction, transcription factors and cytokines. CONCLUSION: Ketamine can induce the significant alteration of gene expression profile of thalamus in rats. [Keywords] ketamine; gene chip; gene expression profile; thalamus [Abstract] Objective: The study of DNA microarray gene expression profile of ketamine anesthesia in rat thalamus changes. Methods: SD rats were 4, divided into control group (n = 2) and the ketamine group (n = 2). Two groups of rats intraperitoneal injection of normal saline, respectively, or 100 mg / kg ketamine. 1 h after taking the hypothalamu