Linear dynamics characterization of L-phenylalanine on rabbit intestinal mucosa alkaline phosphatase inhibition.docVIP

 PAGE \* MERGEFORMAT 5 Linear dynamics characterization of L-phenylalanine on rabbit intestinal mucosa alkaline phosphatase inhibition [Abstract] Objective: To study the linear dynamics characterization of L  phenylalanine on rabbit intestinal mucosa alkaline phosphatase inhibition reliability. METHODS: The alkaline hydrolysis of p-nitrophenyl phosphate (PNPP) initial velocity of product generation. with two substrate concentrations, Michaelis constant calibration of the specific activity calculation (Km) and maximum reaction velocity (Vm), and then, according to the two pairs of inhibitors, the response to determine the concentration of inhibitor type and inhibition constants. Results : Double-reciprocal analysis repeated measurements found that 70% of the Km and Vm with the inhibitor concentration and the simultaneous decline in two changes of kinetic parameters of the ratio of the corresponding inhibition constant (2.4 ± 0.6, n = 9). linear dynamics slightly higher accuracy obtained parameters, Km and Vm with the inhibitor concentration decreased while the emergence rate of about 80%, 2 inhibition constant ratio (2.0 ± 0.8, n = 10), two parameters correspond to the inhibition constants and two inhibitory constant ratio is consistent with the double-reciprocal analysis. such as allowing two inhibition constants of 2.5 times the ratio of fluctuating within, then the two methods indicate that this phenylalanine L  anti-competitive inhibitor of alkaline phosphatase. Conclusion : This linear dynamics method can be used for characterization of anti-competitive inhibitor. [Keywords:] L  phenylalanine; anti-competitive inhibition; alkaline phosphatase; linear dynamics method; inhibition constant 0 Introduction Anti-competitive inhibitor for clinical medicine there are obvious advantages [1-2]. Looking for anti-competitive inhibitor of enzyme required to determine kinetic parameters (Km and Vm) response to the inhibitor concentration to determine the inhi