Local IL10 gene therapy of autoimmune inner ear sensorineural hearing loss in experimental study.docVIP

 PAGE \* MERGEFORMAT 21 Local IL10 gene therapy of autoimmune inner ear sensorineural hearing loss in experimental study Of: Chen Wenbo, Tan Changjiang, Dong Wei Da, Li Yujin, Cai Wenjun Guo waves, Li Wangwei [Abstract] Objective: To explore the topical application of IL 10 gene with the recombinant lentiviral vector on experimental autoimmune sensorineural hearing loss (ASNHL therapeutic effect. Methods: Purification of guinea pigs immunized with the inner ear, causing ASNHL animal model, then The recombinant vectors were treated with tympani, endolymphatic sac and the round window niche of local injection or infiltration, and inner ear hearing function observed morphological changes, both by immunohistochemistry test to slow the virus transfection and gene products in the distribution of the inner ear. Results : ABR wave before and after treatment the mean contrast threshold analysis showed that the experimental group were lower after local gene therapy, the experimental groups after treatment, differences between groups were not significant. lentiviral transfer part of it is the main blood vessel pattern, Corti device , worm shaft and the small blood vessels around the endolymphatic sac, etc.. gene product expression site and transfected into the same site. Conclusion: The recombinant vector was transferred into the inner ear may be different ways, and the expression of gene products in the inner ear, to play an effective ASNHL treatment. [Keywords:] autoimmune sensorineural hearing loss, gene therapy, lentiviral vector [Abstract] Objective: To evaluate therapeutic effects of transferring recombinant replication defective lentiviral vector of interleukin IL 10 gene with GFP marked into inner ear for locally treating experimental autoimmune sensorineural hearing loss (ASNHL) in guinea pig.Methods: Guinea pigs were immunized with 58 kD purified conspecific inner ear antigen and caused animal models of autoimmune sensorineural hearing loss.The expe