MAPK phosphorylation in the way insulin-like growth factor 1 promotion of human myeloma cell line KM3 role in proliferation.doc

 PAGE \* MERGEFORMAT 9 MAPK phosphorylation in the way insulin-like growth factor 1 promotion of human myeloma cell line KM3 role in proliferation Authors: Hua-Fang Wang, Hu Yu, Chun-Yan Sun, Wang Ya-dan Abstract This study investigated the insulin-like growth factor  1 (IGF  1) on human myeloma cell line KM3 proliferation, as well as ways to phosphorylation of MAPK’s role in the process. Detected by flow cytometry with different concentrations of recombinant human IGF  1 treated KM3 cell cycle changes, Western blot detection of intracellular MAPK signal transduction pathway of the main molecule Erk1 / 2 phosphorylation in the IGF  1 stimulation and specifically inhibited by PD98059 changes under the action of, TUNEL assay-specific blocking Erk1 / 2 phosphorylation in KM3 cells in inhibiting proliferation and inducing apoptosis. The results show that, IGF  1 can significantly improve the S phase and G2 / M phase of the KM3 cell ratio and intracellular Erk1 / 2 phosphorylation level of molecules, blocking IGF  1 induced Erk1 / 2 phosphorylation can significantly inhibit the KM3 cell proliferation and cause their apoptosis. Conclusion: IGF  1 induced Erk1 / 2 phosphorylation in the KM3 Molecular cell proliferation plays an important role. Keywords: multiple myeloma Effect of MAPK Signaling Pathway Phosphorylation on Proliferation of Myeloma Cell Line KM3 by Insulin  like Growth Factor 1 Abstract In order to study the effect of insulin  like growth factor 1 (IGF  1) on proliferation of myeloma cell line KM3 and the role of MAPK pathway phosphorylation in this process, the cell cycle distribution shift of KM3 after incubation with series concentration of IGF  1 was detected by flow cytometry. Phosphorate  Erk1 / 2, the key molecule of MAPK pathway, was examined by Western blot after KM3 cells being pretreated with or without PD98059, the special inhibitor of Erk1 and Erk2 phosphorylation. The effect of specifically block