MAPK PI3K pathway in snake venom nerve growth factor-induced differentiation of PC12 cells,.doc

 PAGE \* MERGEFORMAT 18 MAPK PI3K pathway in snake venom nerve growth factor-induced differentiation of PC12 cells, Keywords: Nerve growth factor;, PC12 cells;, cell differentiation;, signal transduction Abstract: Objective To study the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) / Akt pathway in snake venom nerve growth factor (NGF)-induced PC12 cell differentiation in the role. Methods Western blot analysis of p44/p42MAPK and Akt phosphorylation levels and the use of MAPK inhibitor PD98059 and the PI3K inhibitor LY294002, was observed on NGF-induced morphological changes of PC12 cells. The results of cobra venom NGF at 10 ng / mL can be induced by PC12 cells grow processes, 100,1000 ng / mL obvious effect in a dose-response relationship; NGF can effectively stimulate p44/p42MAPK, Akt phosphorylation; specific inhibitors were used to PD98059 inhibited MAPK activity, LY294002 inhibited PI3K activity in post-, NGF-induced cell differentiation are subject to rejection. Conclusion snake venom nerve growth factor-induced PC12 cell differentiation associated with the p44/p42MAPK and PI3K/Akt way. Keywords: Nerve growth factor; PC12 cells; cell differentiation; signal transduction ABSTRACT: ObjectiveTo investigate the role of mitogenactivated protein kinase (MAPK) and phosphoinositide 3kinase (PI3K) / Akt pathways in differentiation of PC12 cells induced by nerve growth factor (NGF) from venom. Methods The phosphorylation of p44/p42MAPK and Akt were detected by Western blot. The role of MAPK and PI3K in the cellular action of PVC12 cells induced by NGF were assessed by use MAPK and PI3K specific inhibitor PD98059 and LY294002 respectively. Results NGF from cobra venom can stimulate p44/p42MAPK and Akt phosphorylation and induce the differentiation of PC12 cells from 10 ng / mL to 1000 ng / mL. The differentiation of PC12 cells induced by NGF was inhibited when MAPK or PI3K pathway was abolished by the spec