Marine microalgae culture medium dimethyl sulfide and dimethyl sulfide acid in the simultaneous analysis.doc

 PAGE \* MERGEFORMAT 19 Marine microalgae culture medium dimethyl sulfide and dimethyl sulfide acid in the simultaneous analysis Of: Zhoucheng Xu Xu Jilin Yan Xiaojun Jiang Ying Hou Yundan [Abstract] water-based DMS (DMS) in the solid phase micro extraction gas chromatography mass spectrometry analysis, the establishment of marine microalgae culture medium DMS, dimethyl sulfide dissolved acid (DMSPd) and particulate dimethyl sulfide acid (DMSPp) synchronization method. The results show that: The method in 0.01 ~ 10.0 μg / L range of good linearity, the detection limit of 10.6 pg, the recovery rate was (93.1 + -1.3)% ; relative error of less than 5%. microalgae culture medium to 4 high-speed centrifugation, the upper and lower solution, solid NaCl were added immediately after the determination of DMS, adding NaOH solution, measured after 12 h reaction of DMS content, according to their numerical difference can be medium by the algae content of DMS and DMSP. detection results reported in the literature with similar conclusions: dinoflagellates dinoflagellates and is scheduled to DMS (P) of the major producers. which coccolithophorid (Pleurochrysis carterae) unit cell DMSPp detection content (13.8 + -0.9) pg / cell [Keywords:] of marine microalgae, the culture medium, dimethyl sulfide, dimethyl sulfide acid, solid phase micro-extraction gas chromatography mass spectrometry 1 Introduction Dimethyl sulfide (DMS) is the most abundant marine volatile sulfur compounds [1,2], through air-sea exchange into the atmosphere, thus affecting the local climate and even the waters of the formation of acid rain, acid fog, etc. [3,4 ]. It is mainly from marine microalgae, and its precursor is dimethyl sulfide propionate (DMSP), DMSP production capacity of marine microalgae species and in which its waters and different [4 ~ 6]. marine micro algal DMSP and DMS in the culture medium of change, to improve the marine environment, especially marine atmosphere of great si