MDA in liver tissues Improved Determination.doc

 PAGE \* MERGEFORMAT 12 MDA in liver tissues Improved Determination [Abstract] Objective To study the carbohydrate on liver malondialdehyde (MDA determination and to improve the determination of the interference. Methods to the sugar content of the different experimental mouse liver homogenate as the experimental material, and by glucose in the 450nm absorbance at 532nm positive correlation between the establishment of linear regression equation, corrected MDA absorbance at 532nm at. calculate the MDA content in the sample. The results of saccharides in liver tissue may interfere with the determination of MDA, especially when the sample difference between sugar content is high, the interference of sugar on the body may cause damage to the extent of the error evaluation. Conclusion The improved method is more scientific and accurate, can be applied to animal liver tissue MDA content. [Keywords:] MDA, Ganoderma lucidum polysaccharides, glucose, linear regression MDA (Malomdialdehvde, MDA is the body of oxygen free radicals attack membrane polyunsaturated fatty acids in the form of lipid peroxide, the amount of MDA test can reflect the body’s level of lipid peroxidation, indirectly reflected the degree of cell damage [1]. The traditional determination method is the use of peroxide degradation products in the MDA and thiobarbituric acid (TBA under acidic conditions at high temperature and condensation to form a red product of the top three Chuan (3, 5,5 - trimethyl oxazole 2,4 - dione in the maximum absorption peak at 532nm to be determined. This approach is often subject to a variety of substances with different degrees of interference [2]. The most important factor is the organization of interference the soluble sugar. animal liver contains large amounts of liver glycogen, in the determination of the degradation would interfere with the determination of glucose. And of animal tissue MDA in the determination of saccharides and elimination of interference