Measurement and Analysis of blood reticulocyte.doc

 PAGE \* MERGEFORMAT 7 Measurement and Analysis of blood reticulocyte Absolute reticulocyte count and percentage is still the most common hematologic reflect the proliferative activity of red blood cell indices, but with the blood cell analyzer in the use of new technologies, many new parameters are found, the instrument not only released from the bone marrow the number of reticulocytes, peripheral blood can also provide the maturity of reticulocytes. To investigate the absolute reticulocyte count, reticulocyte percentage, and the maturity of reticulocytes sensitivity, I use before and after erythropoietin observations, are reported below. 1 Determination of blood reticulocyte 1.1 Determination of blood reticulocyte not only on the blood system disease diagnosis, treatment and observation is of great significance. But also through the observation of reticulocytes, we can understand cancer patients after chemotherapy and chemotherapy, bone marrow changes in the situation. MFR After the evaluation of bone marrow suppression began to recover the more sensitive indicators. MFR + HFR is to estimate the hematopoietic recovery after transplantation of early indicators. Monitoring Network Ret can understand bone marrow transplantation, renal transplant recipients erythropoietin (EPO’s efficacy, dose reduction and treatment early indicator of the program. 1.2 SYSMEX-R3500 has the advantage of directly measured using whole blood reticulocytes, simple, fast, and SE9000, SP100 automatic production dyeing machine, computer workstations together, constitute the HST pipeline. Intraassay CV of its quality control substance 2.95 % intra-sample CV was 4.42% (1.27 ~ 10.05%), consistent with those reported. batches between the CV was 3.56%, consistent with those reported. At the same time the instrument displays the scatter plot directly reflects the maturity of reticulocytes, according to fluorescence intensity of the reticulocyte into high, medium and low fl