Mechanism of sports fatigue Cistanche Experimental Analysis.docVIP

 PAGE \* MERGEFORMAT 14 Mechanism of sports fatigue Cistanche Experimental Analysis [Abstract]: Objective To observe the swimming deserticola on lactate dehydrogenase isoenzymes in mouse liver, glycogen and nitric oxide synthase 3 (NOS3) and to explore the role of sports fatigue mechanism. Methods Kunming mice were randomly divided into normal control group, movement control and experimental groups of Cistanche. normal control group and exercise control group fed normal saline per day 0.2 mL, each experimental group Cistanche deserticola decoction daily gavage 0.2 mL (3 g / kg), a total of 15 d, 1 h after the last administration, exercise control group and experimental group of mice tail Cistanche 5% weight to swim 90 min, 10 h after the mice were killed, together with the normal control group, the liver, some using Preparation of neutral formalin-fixed paraffin section, part of the liver cells made detection of lactate dehydrogenase activity in homogenate, routine HE staining of liver structure, liver glycogen staining showed glycogen, SP immunohistochemical staining mouse liver NOS3 expression. Results exercise control group and the normal control group, high lactate dehydrogenase activity (P lt;0.05), severe structural damage the liver, glycogen content decreased, NOS3 expression decreased (P lt;0.05), the experimental group and exercise control deserticola group, the lactate dehydrogenase activity decreased, mainly lactate dehydrogenase 5 (LDH5) decreased hepatic lobule structure is more clear, glycogen rich, NOS3 expression increased (P lt;0.05). Conclusion deserticola reduce LDH5 activity, increased NOS3 expression, and promote glycogen synthesis, the liver plays the role of protection and promotion of physical recovery. [Keywords:] Cistanche 3 nitric oxide synthase in liver glycogen in mice Abstract: Objective To study the effect of cistanche deserticola on lactate dehydrogenase (LDH) isoenzyme, glycogen and nitric oxide synthase 3 (NOS3) of liv