Medicinal herbs on different sources of saponin content compared Pulsatilla.doc

 PAGE \* MERGEFORMAT 8 Medicinal herbs on different sources of saponin content compared Pulsatilla Of: when the dimension of static, Hu Yuejin, Wang Haixia, Tan Zhijing [Abstract] Objective B4 Pulsatilla saponin as an indicator to study the quality of Pulsatilla different sources for the processing and selection of Pulsatilla to provide information. Method using high performance liquid chromatography, were purchased from Bozhou Chinese herbal medicine and the largest market Pulsatilla Pieces 5 samples and determine the content of Chuzhou wild Pulsatilla. results in the range 0.312 5 ~ 10 μg good linearity, the regression equation Y = 276 350X-12 180, R2 = 0.999 6. Conclusion herbs Pulsatilla saponin from different sources B4 content was larger. [Keywords:] Pulsatilla, origin, Pulsatilla saponin B4, determination; high performance liquid chromatography Keywords:: Pulsatilla chinensis, original place pulchinenoside B4, content determination, HPLC Ranunculaceae Pulsatilla Pulsatilla is Pulsatilla chinensis (Bge.) Regel dry root [1], is a perennial herb, with heat and detoxifying, cooling Zhili, dampness insecticidal effect. Currently on the market products based on wild collection , due to different origin, processing and Pieces of cutting methods, on the effective components in greater impact. The trial of Chinese herbal medicines from the Bozhou stalls big market share of different herbs Pulsatilla 3, 2 Pieces, collecting nine Chuzhou Fengyang Mountain wild Pulsatilla, total 6 samples. to Pulsatilla saponin B4 as reference, using high performance liquid chromatography and its content, for the processing and selection of Pulsatilla reference. 1 Materials American Waters 1525 high performance liquid chromatography, 2487 DAD UV detector, Breeze chromatography workstation (U.S. Waters), TU-1810SPC UV-visible spectrophotometer (Beijing General Analysis General Co), BS21S precision electronic balance (Sartorius, Germany Balance Co., Ltd.). Pulsa