Mesenchymal stem cells differentiate into endothelial cells in vitro.doc

 PAGE \* MERGEFORMAT 4 Mesenchymal stem cells differentiate into endothelial cells in vitro Author: Si-Yuan Yang, Dian Ke, Shi Ying Kang, Huan-Wen Chen, Meng Wei [Abstract] Objective: To search for cardiovascular tissue engineering a new source of seed cells to explore the rat bone marrow-derived mesenchymal stem cells (MSCs) in vitro methods and to the vascular endothelial cell differentiation capacity. Methods: Using lymphocyte separation medium to isolate MSCs, and in vitro amplification, laser scanning confocal microscopy detection of surface antigen expression, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) in inducing differentiation medium directed induction passage so that the direction of differentiation to vascular endothelial cells to observe the changes in cell morphology, detection of endothelial cell-specific marker expression. Results: MSCs in vivo rumor on behalf of the amplified laser confocal microscopy showed that CD44, CD90 expression was positive, CD31, CD45 negative, differentiated endothelial cells after the cells have the morphological characteristics and expression of endothelial cell-specific markers CD31. Conclusion: Bone marrow-derived mesenchymal stem cells can be induced in vitro differentiation into endothelial cells. [Keywords:] bone marrow; stem cell transplantation; rat Spragua-Dawley; mesenchymal stem cells; induced differentiation; endothelial cell [Abstract] Objective: To explore methods of separating and culturing mesenchymal stem cells (MSCs) of rats in vitro and to study the differentiation capability of bone marrow MSCs into endothelial cells, so as to provide new source of seed cells for tissue engineering. Methods : MSCs were separated from bone marrow with density gradient centrifugation and wall sticking screening, and amplified in vitro. The surface antigens of MSCs were evaluated with confocal laser scanning microscope (CLSM). The passage cells were induced by differe