Methylation in human cervical cancer cell lines FHIT gene regulation.doc

 PAGE \* MERGEFORMAT 2 Methylation in human cervical cancer cell lines FHIT gene regulation [Abstract] Objective To investigate the occurrence of FHIT in cervical cancer and the role of the FHIT gene inactivation mechanism. Method of cervical cancer cells in culture C  33A, HeLa, CasKi, SiHa, and human umbilical vein endothelial cells ECV  304, for 5  aza  dC interventions before and after the FHIT gene in five cells, methylation analysis and by reverse transcription Polymerase chain reaction and immunofluorescence staining to detect 5  aza  dC interventions before and after the FHIT gene in five cells. Results There were four kinds of cancer cells, methylation of FHIT gene in normal control cells, ECV  304 in the 5  aza  dC before and after intervention were no significant FHIT gene amplification products. Four cervical cancer cells in the 5  aza  dC chemical intervention in the former were no significant FHIT fluorescent coloring, while the cytoplasm after the intervention of the visible intensity of FHIT expression markedly enhanced, especially in the 10  6M and 2 * 10  6M interfere with the expression of the concentration of the strongest (P