Microencapsulation of antioxidants catechin and its effect on the kidney in rats.docVIP

 PAGE \* MERGEFORMAT 21 Microencapsulation of antioxidants catechin and its effect on the kidney in rats Authors: Yun Tian, Xiang-Yang Lu, Xiao-Jie He, Xiao-Bo, Peng Lisha, Zhu-Wen Yi, LUO Ze-min 【Abstract】 Objective To study the antioxidant activity of catechin, catechin microcapsule preparation technology and its impact on the efficacy of nephrotic syndrome in rats. Chemiluminescence method using natural plant antioxidant capacity of scavenging reactive oxygen species; use Spray Drying catechin microcapsule; through acute toxicity and sub-chronic toxicity tests evaluate the safety of micro-capsules; established model of adriamycin-induced nephrotic syndrome in rats by using Coomassie brilliant blue method measured the amount of urinary protein excretion, were assessed by semi-quantitative scoring of glomerular, tubulointerstitial pathological changes. Results catechin, rosemary extract and grape seed extract removed IC50, respectively 6.16,184.29 and 15.86μg/mL; remove OH in IC50 were 0.59,39.89, and 2.93μg/ml; remove H2O2 in IC50 were To 0.69,16.80 and 1.34μg/ml, description catechin scavenging reactive oxygen capacity than rosemary extracts and grape seed extract strong; Spray Drying excellent performance of catechin microcapsule of the main process parameters : solids content of 25%, the core material and wall material ratio of 6:4, ethyl cellulose and PEG ratio of 8:2; catechin microcapsule are real non-toxic level, non-toxic side-effects; Animal model experiments in rats with nephrotic syndrome at the end, the groups of different treatments 24h urinary protein excretion in rats were as follows: nephropathy group “catechin treatment group,” hormone therapy group “catechin microcapsule treated” control group; kidney Li points were as follows: nephropathy group “catechin treatment group,” catechin microcapsule treatment group, “hormone therapy group” control group. 24h urinary protein excretion and renal pathological score was positively correlate