Microwave assisted extraction of polysaccharides chuanxiong.doc

 PAGE \* MERGEFORMAT 7 Microwave assisted extraction of polysaccharides chuanxiong [Abstract] Objective To determine the conditions in the microwave extraction of polysaccharides from the optimal conditions Chuanxiong. Methods of single factor experiments and orthogonal test, the microwave power, solid to liquid ratio, extraction time factors, each of three levels, select the L9 (34) orthogonal, anthrone - sulfuric acid Determination of polysaccharide content. Results optimum conditions for the microwave power 231W, liquid ratio 1:40, extraction time 10 min, polysaccharides under these conditions was 3.06%. Conclusion microwave extraction time is short Chuanxiong polysaccharide, extraction efficiency, and has broad application prospects. [Keywords:] Chuanxiong, microwave extraction, polysaccharides, single-factor experimental design, orthogonal Chuan Xiong Ligusticum chuanxiong Hort. Is a common authentic traditional Chinese medicine in Sichuan, with blood circulation of qi, chills and pain relief, Gloomy dampness and other effects, clinically used to treat headache, dizziness, Fengshibitong, traumatic swelling, pain, menstrual Stagnation is not transferred and the postpartum abdominal pain and other symptoms [1]. In this study, microwave extraction of polysaccharides Chuanxiong to explore the factors that affect the extraction to determine the optimum condition for Chuanxiong polysaccharides provide some basis for further research. 1 apparatus and materials 752 UV spectrophotometer (Shanghai Spectrum Instruments Co., Ltd., WD700TL17 3 microwave oven (Glanz Co., Ltd. Guangzhou, Centrifuge 5810R refrigerated centrifuge (Eppendorf U.S. companies, electronic balance BS200S (Beijing Saiduo Li Ping Limited. Chinese herbal medicine market in Chengdu Chuanxiong purchased by the Institute of Pharmaceutical Engineering, the identification of Dr. Tang Can, dry grinding, 40 mesh sieve and set aside, glucose, ethanol, sulfuric acid, anthrone (all analyt