Modified Shibahira Granule Extraction Process.doc

 PAGE \* MERGEFORMAT 7 Modified Shibahira Granule Extraction Process Author: Pan Jinhui Chen Yue Peng Yao Kwok Shing Chan Chun Fai [Abstract] Objective: To study the Modified Shibahira granules of the extraction process, provide a basis for determining its efficacy. Methods: orthogonal design to baicalin transfer rate and the dry extract yield of the indexes, examining ethanol concentration, extraction time, extraction times and ethanol by adding the amount of four factors on the extraction. Results: After a comprehensive analysis, the best extraction conditions are: A2B3C3 that increased 11-fold 65% ethanol, reflux extraction two times, each time 2h. Conclusion: The extraction conditions were stable, reasonable, and can reduce production costs, in order to provide the basis for large-scale production preparation. [Keywords:] Modified Shibahira granules; orthogonal experiment; extraction process; baicalin Modified Shibahira is my casual name of the old provincial hospital clinical medicine prescription, because the clinical effect is good, deeply welcomed by the majority of patients, in order to facilitate the patient’s use, consider a new formulation developed into granules, easy to carry patients, use, storage. We baicalin transfer rate and the yield of dry extract Technology study factors. By orthogonal test to optimize the process conditions, choose a reasonable extraction conditions. A reagent and instrument Japanese wood Shimadzu C 5  930 TLC scanner (Shimadzu on wood), MA 110 electronic analytical balance (Shanghai the next day flat Instrument Factory), 2K  82B-type vacuum drying oven (Shanghai Experimental Instrument Factory), TN  1008-type tray torsion balance (Shanghai Balance Instrument Factory No. 1). Baicalin reference substance (Lot: 120614  200512, from China to provide pharmaceutical and biological products); skullcap, Bupleurum, Licorice, etc. provided by the Department of Pharmacy, Zhanjiang first hospital of Chinese medicine.