Modified SDS method of taking cannabis days Draba DNA Extraction.doc

 PAGE \* MERGEFORMAT 13 Modified SDS method of taking cannabis days Draba DNA Extraction Keywords: Gastrodia elata; genomic DNA; DNA extraction Modified SDS method of taking cannabis, Draba DNA Extraction days * 【Abstract】 days Gentiana Draba as material, a modified SDS method Gastrodia DNA extraction was studied by UV spectrophotometer and agarose gel electrophoresis, can get high-purity DNA, OD260/OD280 1.75 ~ 1.9 between the proteins, polysaccharides, RNA and so more thorough removal, suitable for further analysis. Keywords: Gastrodia elata; genomic DNA; DNA extraction Study on extraction of DNA of Gastrodia elata.scape in an improved SDS method 【Abstract】 The DNA of Gastrodia elata.scape were extracted with an improved SDS method.The extracted DNA was examined by ultraviolet absorption test and agarose gelelectrophoresis.The results showed that the improved SDS method was suitable for DNA extraction in Gastrodia elata.The purity of the extracted DNA was very high with OD260/OD280 value of 1.75 ~ 1.9.The protein, polyhexose and RNA were eliminated completely.It could be used for analysis. 【Keywords:】 gastrodia elata; genome DNA; DNA extracting Draba from medicinal cannabis extract to the days of high-quality DNA, is successfully constructed DNA fingerprinting premise. Effective removal of DNA impurities (sugars, phenols, etc.), to avoid the polysaccharides, polyphenols, pigments, etc. affect the DNA polymerase activity is the key to obtaining high quality DNA. Recently, the total DNA extraction of plant, there are two main methods: CTAB method and SDS method. Since this experiment the selected material as fresh flowers in these young Gastrodia Draba, one to avoid the degradation of DNA molecules, and second, these young days as fresh cannabis Draba part of DNA growing point of its high content of secondary metabolites and less to avoid the multi - sugar and phenolic impurities affect the DNA p