Monascus Purification and identification of strains.doc

 PAGE \* MERGEFORMAT 7 Monascus Purification and identification of strains [Abstract] [Objective] in the red on red fermented bean curd for isolation and purification and identification of aspergillosis. [Methods] According to the Monascus eosinophils, anti-growth characteristics of ethanol, with ethanol-containing wort containing lactic acid separation of red rot of rice milk in medium red aspergillosis, and with the number of dilution-plate method for purification. Purified Monascus microscopic observations to determine their genus other. MEA medium in accordance with its 25 ℃ cultured colony morphology characterization to the species. [Results] isolated from the red bean curd 2 strain was identified M-1 is a bundle hair red aspergillosis (M.pilosus), M-3 is a red yeast Monascus (M.anka). [Conclusion] The red bean curd isolated pure culture of red yeast fungi. [Keywords:] Red aspergillosis; separation; purification; identification Abstract: [Objective] To isolate, purify and identify Monascus strains from red furu. [Method] Based on eosinophilic and ethanol tolerant characteristics of Monascus, stains were isolated from red furu by culturing on malt juice medium containing alcohol and rice medium containing lactic acid . Strains were purified using logarithmic plate dilution method, and then determined genera according to their micro-morphological characteristics.By culturing on malt extract agar medium with temperature of 25 degrees Celsius, strains were identified to species based on the morphological characteristics of colonies. [ Result] M-1strain isolated from red furu was identified as Monascus pilosus and M-2 Monascus anka. [Conclusion] Strains isolated from red furn are Monascus fungi. Keywords:: Monascus; isolation; purification; identification Monascus fungi under (Eumycophyta) Ascomycota (Ascomycota) true Ascomycetes (Euascomycetes) Bulk Order ascomycetes (Eurotiales) Red aspergillosis Division (Monascaceae) red yeast genus (Monascus) [1],