Mothman Kidney Capsule Determination of icariin.doc

 PAGE \* MERGEFORMAT 5 Mothman Kidney Capsule Determination of icariin Author: Xiu-Yun Zhang Yu Jiang Bo Chong-Lin Yang Mei LIU Shi-yue [Abstract] Objective: To establish a high-performance liquid chromatography Mothman Bushen Capsule icariin method. Methods: The high-performance liquid chromatography (HPLC), Diamonsil C18 column, mobile phase of acetonitrile - water (30:70), detection wavelength of 270nm. Results: In the 0.172μ g ~ 0.860μ g icariin showed good linear relationship within the framework of (r = 0.9999, n = 5), the average recovery was 98.43%, RSD = 0.81% (n = 5). Conclusion: The method is simple, accurate and reproducible and can be used Mothman Kidney capsule quality control. [Keywords:] Mothman Kidney capsule; icariin; high-performance liquid chromatography Mothman Kidney Capsule from the male oak silk moth, Epimedium, Rehmannia glutinosa, Cistanche, Achyranthes composed of 14 Chinese herbal medicines. Kidney impotence, Qi Pei Yuan. Applicable to kidney yang deficiency syndrome, Zheng Jian Shenpi fatigue, Wei Han cold extremities, Yaoxisuanruan, impotence and so on. Epimedium for Fang major Medicine Smell, its quality control is very important, it established a high-performance liquid chromatography method of icariin content, control Mothman kidney capsule quality. An experimental part of the 1.1 The equipment, reagents, sample LC  10ATVP high-performance liquid chromatograph, SPD  10AVP UV detector (Shimadzu); N2010 chromatography data workstation (Zhejiang University Intelligent Information Engineering Institute). Chromatography pure acetonitrile, water re-distilled water, other reagents were of analytical grade. Icariin reference substance (Chinese medicines and biological products), hawk moths have kidney capsule (Yanbian Jilin Aodong Pharmaceutical Co., Ltd., batch number: 041201,041202,041203,041204,041205). 1.2 Chromatographic conditions  water-acetonitrile (30:70) as mobile phase; detection wavelength 270nm; flow rate of 1.