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Molecular Biotechnology in Traditional Chinese Medicine Identification
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Molecular Biotechnology in Traditional Chinese Medicine Identification
[Abstract] molecular biology and botany, zoology of the combination of the varieties of traditional Chinese medicine into a new level. Chinese herbal medicines (excluding mineral medicine depend on the diversity of biological resources is based on its gene polymorphism The results, while the polymorphism can be detected at the molecular level, it is than in the form, organization, chemistry is more representative of the level of detection of the type of variation of genetic markers.
[Key Words] molecular biotechnology, gene technology, medicine identification
In recent years, molecular biotechnology and botany, zoology of the combination of the varieties of traditional Chinese medicine into a new level. Chinese herbal medicines (excluding mineral medicine depend on the diversity of biological resources is based on the results of its gene polymorphism , and gene polymorphism can be detected at the molecular level, it is than in the form, organization, and chemical testing on the level of variation is more representative of the types of genetic markers. At present, the identification of molecular biology used in Chinese medicine methods are: Random amplified polymorphic DNA, DNA direct sequencing, restriction fragment length polymorphism analysis, polymerase chain reaction sequencing technology, bio-chip technology, DNA fingerprinting technology.
1 gene technology
1.1 DNA molecular genetic marker technology [1]
This is a different individual organisms according to differences in the genetic material DNA methods to identify species. The appearance of biological traits, morphology, structure, chemical composition, protein and other effects not only by genetic factors, but also the growth and biological environment developmental stages, physiological states. and a direct DNA as the carrier of genetic information, subject to the above factors, the identific
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