Molecular marker technology in traditional Chinese medicine classification and resource protection Application Progress.doc

 PAGE \* MERGEFORMAT 23 Molecular marker technology in traditional Chinese medicine classification and resource protection Application Progress Authors: GUO Bao-lin Liu Wan-shui CHEN Yu-ting Wang Jianhui Zhu Man-ping Wang Danhong [Keywords:] traditional Chinese medicine classification of molecular marker techniques Resource Protection Molecular marker technology generally refers to the study of nucleic acid and protein molecules such as carrying the genetic information of the characteristics of technology, molecular markers can be divided into DNA molecular markers and protein molecular markers. Molecular marker technology into the field after being widely used in traditional Chinese medicine classification, identification and protection at all levels by more and more attention. This article will focus on resource conservation in traditional Chinese medicine classification and application of IT. 1 DNA molecular markers DNA molecular markers, with the development of nucleic acid molecular techniques developed have been applied to various fields of life sciences, especially in the system evolution, conservation genetics, species identification and seed selection, genetic map construction, gene mapping, etc. has been widely applied. According to DNA molecular markers used in the core technology in general can be divided into three categories: First, molecular hybridization-based DNA fingerprinting techniques; 2 is a PCR-based DNA fingerprinting techniques; third DNA sequence markers. 1.1 based on molecular hybridization of DNA fingerprinting technology is based on molecular hybridization of DNA fingerprinting technology can be divided into RFLP, SSR and other technologies of the experiment generally include restriction enzyme digestion, Southern mark, probe mark, molecular hybridization experiments such as cumbersome steps, and only the length of the scope probe to detect endonuclease recognition sites of variation, polymorphism detection efficiency is low,