Moderate copper deficiency on the rat carotid artery after endothelial denudation MMP.docVIP

 PAGE \* MERGEFORMAT 13 Moderate copper deficiency on the rat carotid artery after endothelial denudation MMP Authors: ZHOU Jing-yu, GUO Wen-Yi, Hong-Zhi Zhou, Guo-Liang Jia, Rong-Qing Zhang [Keywords:] matrix metalloproteinase Influence of moderate copper deficiency on expression and activity of MMP9 and restenosis in ballooninjured rat carotid arteries [Abstract] AIM: To study the influence of moderate copper deficiency induced by tetrathiomolybdate (TM) on expression and activity of matrix metalloproteinases9 (MMP9) and restenosis in ballooninjured rat carotid arteries. METHODS: Male SpragueDawley rat were prefed distilled water or TM at a dosage of 10 mg / kg 2 weeks before balloon injury. Plasma ceruloplasmin levels were monitored, and the oral administration of TM could rapidly reduce and sustain the plasma level of ceruloplasmin in all TMtreated animals at a 70% -80% level of reduction after 2 weeks. All rats were subjected to balloon injury and then were killed at 1, 3, 7 and 14 d. TMtreated rats were administrated TM daily after injury. The extent of neointimal thickening was evaluated. Western blot and gelatinzymography were used to detect the expression and activity of MMP9. RESULTS: Compared with the control group, the extent of neointimal hyperplasia in TMtreated rats was significantly reduced 2 weeks after injury. The expression and activity of MMP9 were lower significantly in TMtreated rats than those in control rats from 1 to 7 d (P amp;lt;0.01). CONCLUSION: The oral administration of the Cu2 chelator TM could reduce neointimal thickening after balloon injury in the rat. And the expression and activity of MMP9 were significantly suppressed by moderate copper deficiency induced by TM. [Keywords] matrix metalloproteinases9; copper; tetrathiomolybdate; restenosis [Abstract] Objective: To explore the four sulfur molybdate (TM) caused by moderate copper deficiency on the rat carotid artery intimal hyperplasia after balloon injury and matrix met