Mouse Wnt.doc

 PAGE \* MERGEFORMAT 8 Mouse Wnt Authors: Zong-Sheng Yin, Zhang Hui, Sheng-Quan Zhang, Wang Wei, Huaxing 1, Yong Hu, Li Guangwu [Keywords:] Wnt Expression and biological activity of mouse Wnt3a gene in NIH3T3 cells [Abstract] AIM: To express recombinant mouse Wnt3a signal protein with biological activity. METHODS: The recombinant eukaryotic expression plasmid, pSecTag2/Hygro BWnt3a, was transfected into NIH3T3 cells by liposome, then the expressed protein was detected by Western Blot. The saturation density and the capability of suppressing apoptosis in Wnt3a/NIH3T3 cells were evaluated. RESULTS: The Wnt3a signal protein was stably expressed in Wnt3a/NIH3T3 cells. The saturation density and the capability of suppressing apoptosis in Wnt3a/NIH3T3 cells were markedly increased. CONCLOUSION: We successfully construct the recombinant Wnt3a signal protein with biological activity. [Keywords] Wnt3a; eukaryotic expression; contact inhibition; apoptosis [Abstract] Objective: To express with the biological activity of recombinant mouse Wnt3a signaling protein. Methods: lipofection reagent recombinant eukaryotic expression vector and screened pSecTag2/Hygro BWnt3a transfected NIH3T3 cells with stable expression, Western Blot recombinant Wnt3a protein expression, and cell fusion Wnt3a/NIH3T3 density and the ability to provide detection of anti-apoptosis. Results: Wnt3a signaling protein in the stable expression of Wnt3a/NIH3T3 cells, Wnt3a signaling protein can significantly improve the integration density of NIH3T3 cells and anti - apoptotic capacity. Conclusion: In NIH3T3 cells expressing recombinant Wnt3a signaling proteins with biological activity. [Keywords:] Wnt3a; eukaryotic expression; contact inhibition; apoptosis 0 Introduction Mouse Wnt genes Wnt3a gene is an important member of the family and its encoded protein expression of Wnt3a signal. Wnt3a signaling proteins can activate the classic Wnt / β catenin signaling pathway. Wnt3a signaling protei