Mouse mesenchymal stem cells amplified biological characteristics of.doc

 PAGE \* MERGEFORMAT 26 Mouse mesenchymal stem cells amplified biological characteristics of Authors: Xie Lena, Wang Min, Qiu Huiying, Gao L, Zhou Hong, Gong Sheng-lan Abstract The purpose of this study is the separation and enrichment of mouse bone marrow-derived mesenchymal stem cells (mMSC), identification of its biological characteristics and multi-differentiation potential. Take 4-5 week-old male C57BL / 6 mouse bone marrow cells, using the whole law and monoclonal bone marrow adherent culture method separation, purification and amplification mMSC; analysis of cell-mediated immunity phenotype, growth curve, cell cycle; multi-differentiation the potential identification; subcultured for 30 generations, carried out into the tumor detection. The results showed that: the establishment of the mouse mesenchymal stem cell lines in vitro can be continuously subcultured for 30 generations, cells remain multi-differentiation potential of cells highly expressed CD29, CD44, Sca  1, MHC  Ⅰ, moderate expression of CD13, CD90.2, did not express CD117, CD45, Flk  1, MHC  Ⅱ antigen. mMSC in vitro can be induced to differentiate into bone, fat, cartilage cells. Conclusion: All bone marrow adherent law and enrichment of colony culture method available mMSC, its in vitro continuous passage for more than 30 on behalf of the biological characteristics of stability can be maintained, with a high degree of proliferation and multilineage differentiation potential, no tumorigenicity. Keywords: bone marrow mesenchymal stem cell biological characteristics of cell culture Enrichment and Biological Characteristics of Murine Mesenchymal Stem Cells Abstract The study was aimed to isolate and establish mesenchymal stem cell line from adult murine bone marrow as well as to identify its biological characteristics and differentiation potential. Bone marrow cells (BMCs) were collected by flushing the femurs and tibias of 4  5  week  old male C57BL / 6 mi