MRL - lpr murine CD4 + CD25 + regulatory T cells and related genes.doc

 PAGE \* MERGEFORMAT 13 MRL / lpr murine CD4 + CD25 + regulatory T cells and related genes Of: Luxue Hong Tian Geng Yang Wei Hua Li Yi Gu [Abstract] Objective To study the MRL / lpr systemic lupus erythematosus (SLE mice (model group, the thymus, the spleen CD4 + CD25 + regulatory T cells (Tr gene number and the changes of Tr in the pathogenesis of SLE. Methods detected by indirect immunofluorescence model group and control group the level of serum anti-nuclear antibodies and flow cytometry of thymus and spleen in the number of Tr, RT PCR detection of functional genes Tr. Results of model group of anti-nuclear antibodies were positive, while the control group were negative, thymus, spleen Tr quantitative model group and control group no significant difference, Foxp3 expression level of the thymus in the model group and control group no significant difference in the model group, the spleen was lower than the control group, CTLA 4 expression levels in model group were higher than the thymus and spleen. Conclusion Tr changes in the number of MRL / lpr mice can not afford a key role in the pathogenesis, and functional changes of Tr, in particular, the functional defects in peripheral may be the One of the reasons the incidence, as the inhibitory function of Tr really changed, still needs further testing in vitro to determine the inhibitory function. [Keywords:] MRL / lpr mice, systemic lupus erythematosus, CD4 + CD25 + regulatory T cells, mice [Abstract] Objective To investigate CD4 + CD25 + regulatory T cells and target genes in thymus and spleen of MRL / lpr mice. Methods Serum anti nuclear antibody was detected by indirect immunofluorescence. CD4 + CD25 + Tr was determined by flow cytometry and its target genes were detected by RT PCR. Results Anti nuclear antibody was positive in model group but negative in control group. There were no significant differences of CD4 + CD25 + Tr in model and control groups. Foxp3 levels of model group was no signific