Motherwort Leonurine in the HPLC detection of.doc

 PAGE \* MERGEFORMAT 9 Motherwort Leonurine in the HPLC detection of Author: Gong Chang-Qin, Zhou Ling, Mr Wong, Chang-jun, ZHANG Shi-Yu [Abstract] Objective To establish the HPLC Leonurine Motherwort in detection methods. Method C18 column, mobile phase: methanol  pH = 1.7 acetic acid / sodium acetate buffer (volume ratio 85:15), flow rate 0.6 mL * min-1, detection wavelength 277 nm, sensitivity 0.1AUFS, column temperature was 25 ℃ . Results Motherwort Herb Leonurine mass fraction of 0.015%; The average recovery was 100.08%, RSD of 0.15%. Conclusion Direct detection of high-performance liquid chromatography Leonurine, easy, accurate and reliable. [Keywords:] Motherwort; Leonurine; high-performance liquid chromatography Motherwort Motherwort plants for Labiatae (Leonurus heterophyllus Sweet) part of the dry ground in order to flower bud stage, or dried aerial parts of fresh goods, medicine, sexual bitter, Sim, slightly cold, owned by the liver, pericardium, with blood circulation to regulate menstruation, stasis of Health new, shrink the uterus of the efficacy, attending irregular menstruation, Tailou dystocia, abdominal pain and post-deposition resistance via such certificate, known as ‘blood family saint drug’, ‘by producing medicine’ is known. Motherwort main contains alkaloids, diterpenes, sterols, flavonoids, organic acids, fatty acids and other ingredients. Alkaloid in Leonurus Motherwort Stachydrine base and the main [1]. Pharmacological studies have shown that the main active ingredient Leonurus Leonurine (leonurine) have significant direct expansion of peripheral vascular, increase blood flow, such as the role of anti-platelet aggregation activity [2], can effectively lower blood viscosity and increase red blood cell deformability can also regulate menstruation bleeding, protecting myocardial ischemia-reperfusion injury. In the ‘Chinese Pharmacopoeia’ [3] and the level of standards, Motherwort Leonurus herbs, and quality control of preparations