MTT colorimetric assay amygdalin on rabbit lens epithelial cell proliferation.doc

 PAGE \* MERGEFORMAT 8 MTT colorimetric assay amygdalin on rabbit lens epithelial cell proliferation [Abstract] Objective amygdalin (Amygdalin) on cultured rabbit lens epithelial cell proliferation. Methods 2nd generation logarithmic phase in rabbit lens epithelial cells, cultured 24h, with different concentrations of amygdalin ( 4.0,2.0,1.0,0.5,0.25,0.125 mg / L) 24h, observed by MTT amygdalin on proliferation of rabbit lens epithelial cells. The results of amygdalin with the increasing concentration of rabbit lens epithelial cell proliferation inhibition gradually. Conclusion amygdalin can inhibit proliferation of cultured rabbit lens epithelial cell proliferation, the inhibition in a certain range of concentration in a dose dependent manner. [Keywords:] amygdalin; rabbit lens epithelial cells; posterior capsule opacification; MTT assay [Abstract] Objective To study the effects of Amygdalin on the proliferation of rabbit lens epithelial cells (RLECs) in vitro.Methods RLECs were primary cultured.After the second generation of RLECs were incubated for 24 hours, we added different concentraions of Amygdalin for 24 hours.The cell proliferation of RLECs was determined by MTT assay.Results The inhibitory rates of RLECs proliferation were increased in dose.dependent ways by Amygdalin in vitro.Conclusion Amygdalin could effectively inhibit the proliferation of RLECs, and it might have broad prospects in the prevention of posterior capsular opacification. [Keywords:] amygdalin; rabbit lens epithelial cells; posterior apsular opacification; MTT assay Posterior capsular opacity (posterior capsular opacification, PCO) is a modern extracapsular cataract extraction and intraocular lens implantation in the most common complications. Posterior capsule opacification was mainly due to the occurrence of postoperative residual lens epithelial cells (lens epithelial cells, LECs) occurring proliferation and posterior capsule fibrosis. its incidence is often associat