Mycobacterium tuberculosis antigen 85A and GMCSF induced anti-melanoma effect of.doc

 PAGE \* MERGEFORMAT 15 Mycobacterium tuberculosis antigen 85A and GMCSF induced anti-melanoma effect of Of: Liu Chunsheng, Wu Yun, Chun Dou, Wen Ping, Zhao Shu Feng, Tang rights Li Jinlong, Wang Yaqin [Abstract] Objective: Mycobacterium tuberculosis antigen 85A (Ag85A) and cytokine GM CSF, as a heterologous antigen and immune enhancer, research in mice can effectively induce immune responses against melanoma. Methods: Molecular Biology Law recombinant plasmid pRSC Ag85A/GM CSF, obtained by cell transfection Ag85A and GM CSF stable expression of B16 melanoma cells only .48 C57BL / 6 mice were randomly divided into B16 cell group, B16 pRSC cell group, B16 Ag85A cells group and the B16 Ag85A/GM CSF cell group, respectively, the different cells were subcutaneously inoculated B16 mice. by observing the strength of their tumorigenicity, survival time of tumor-bearing mice, rats, serum levels of IFN and CTL, NK cell killing activity, analysis of Ag85A and GM CSF in the role of anti-tumor immune responses. Results: B16 Ag85A/GM CSF cells in mice significantly inhibited tumor growth, the average survival time was longer than the control group 40%, serum IFN secretion than the control group 1 times, CTL, NK cell activity compared with other groups has greatly improved. Conclusion: The stable expression of Ag85A vaccination of B16 cells and GM CSF can reduce tumorigenicity in mice, inducing an effective anti-tumor effect. [Keywords:] Mycobacterium tuberculosis antigen 85A; granulocyte-macrophage colony-stimulating factor; tumorigenicity; adjuvant [Abstract] Objective: To study anti melanoma effect in mice injected with melanoma cells expressing Mycobacterium tuberculosis antigen 85A (Ag85A) and GM CSF as heterogeneity antigen and immunoadjuvants. Methods: The recombinant pRSC Ag85A/GM CSF was constructed by the molecular biological technology and was transfected into the melanoma B16 cells and the transcription of Ag85A and GM CSF in B16 cells was respectiv