Natamycin producing strain genome rearrangement breeding.doc

 PAGE \* MERGEFORMAT 23 Natamycin producing strain genome rearrangement breeding Abstract Streptomyces gilvosporeus SG  1 protoplast mutagenesis and screened by ultraviolet streptomycin resistant strains, to obtain high-yield strains of Natamycin. In the above-mentioned high-yielding mutant strains selected four as the parental genomic rearrangement breeding, screening has been high-yielding strains of rearrangement, in which a rearrangement of the S.gilvosporeus GS  74 strains of natamycin production of 3574mg / L, for the production of the highest parental strains of 153%, than the original parent strain SG  1 increased 1.17-fold. Keywords: Natamycin brown yellow spore genome rearrangement in Streptomyces Natamycin  producing strain breeding by genome shuffling ABSTRACT After isolating the protoplasts of Streptomyces gilvosporeus SG  1, they were mutated with UV irradiation, the streptomycin resistance mutants were screened, and the high natamycin producing strains were obtained. Among them, 4 strains with high natamycin production were selected as parent strains for genome shuffling. Consequently a high natamycin yeilding strain, S.gilvosporeus GS  74, was obtained, whose natamycin productiion reached 3574mg / L that was 153% of the parents strains and higher by 1.17 times than that of the starting strain. KEY WORDS Natamycin; Streptomyces gilvosporeus; Genome shuffling From the Natal Streptomyces (Streptomyces natalensis), Chattanooga Streptomyces (Streptomyces chatanoogensis) and brown yellow spore of Streptomyces (Streptomyces gilvosporeus) generated by fermentation of producing strain such as natamycin (natamycin) is an efficient widely spectrum anti-fungal agent, can effectively inhibit the growth of yeast and mold and prevent filamentous fungi and mycotoxins formation [1]. The U.S. Food and Drug Administration (FDA) and China’s Ministry of Health in 1982 and 1997 has officially approved as a food additiv