Near β titanium alloy micro-arc oxidation treatment on the surface of osteoblasts in the early behavior.doc

 PAGE \* MERGEFORMAT 8 Near β titanium alloy micro-arc oxidation treatment on the surface of osteoblasts in the early behavior Authors: ZHAO Ling Chau, Yu-Mei Zhang, Wei Yanping, Li Jian Xue [Abstract] Objective: To observe the micro-arc oxidation (MAO) method of near β titanium alloy Ti  5Zr  3Sn  5Mo  15Nb (TLM) after surface treatment on the early behavior of osteoblasts. Methods: Using pulsed DC power supply in two different voltages on the TLM surface micro-arc oxidation treatment and surface morphology of the sample with the electron microscope. MTT proliferation of osteoblasts was detected by using commercial kits detect total protein synthesis and alkaline phosphatase (ALP) activity. Results: Micro-arc oxidation treatment can be formed on the surface layer of TLM bioactive porous oxide layer, its morphology and micro-arc oxidation voltage is related to low voltage when the pore structure formed by a smaller, high-voltage large-pore structure formed. osteoblasts In the micro-arc oxidation of the surface of the early proliferation (24,72,120 h) and 7 d total cellular protein synthesis was significantly higher than polished surface, and the cells in the high-voltage micro-arc oxidation is superior to the surface of the proliferation of low-voltage surfaces. and 7 d culture After the micro-arc oxidation of the surface of cells, ALP activity was significantly lower than the polished surface, and the high-voltage surface of the lowest. Conclusion: Micro-arc oxidation treatment changed the surface morphology and other characteristics of TLM, so as to promote osteoblast proliferation and synthesis of its surface secretory activity, but inhibit the ALP activity. formed under different voltage of different micro-arc oxidation morphology of the early behavior of osteoblasts influence. [Keywords:] titanium alloys; micro-arc oxidation; surface properties; osteoblasts 0 Introduction At present, widely used in clinical pure titanium and Ti  6Al 