Neonatal hyperbilirubinemia CMV DNA Detection and significance of.doc

 PAGE \* MERGEFORMAT 6 Neonatal hyperbilirubinemia CMV DNA Detection and significance of Of: Yangling, Sonja cloud, often color-tai, Geng Jianzhong [Keywords:] newborn; hyperbilirubinemia; cytomegalovirus To explore the neonatal hyperbilirubinemia and CMV (cytomegalovirus, CMV) infection, we treated in our hospital, 48 patients with unexplained neonatal hyperbilirubinemia, were CMV DNA in urine tests were positive mothers of children also had CMV DNA in urine testing, now reported below. 1 Subjects and methods 1.1 as Group of children in our hospital from January 2005 to December 2007 admitted 48 patients with unexplained neonatal hyperbilirubinemia. Among them, male 23 cases, 25 cases of female infanticide, birth days of 1 ~ 28 d, are in line with neonatal hyperbilirubinemia in the diagnostic criteria [1], and exclusion of premature birth, asphyxia, hemolytic, pneumonia, omphalitis, breast milk jaundice, biliary abnormalities, and hepatitis virus infection (hepatitis were negative two pairs of such half- cause as hyperbilirubinemia. The control group in our hospital in the production of 48 normal newborns, follow-up confirmed 28 days after birth, hyperbilirubinemia excluded, of which 21 cases of boys, girls 27. Of CMV DNA in urine for more than two positive new mothers, using the same method of detection of CMV DNA in urine. 1.2 Methods 1.2.1 Specimen Collection Children in hospital to take morning urine during the normal newborn urine samples from the parents for sacrificing and sent to the hospital, follow-up, guiding the morning urine specimens from the parents the correct way. The urine of newborn CMV DNA-positive mothers, but also specimens from morning urine for inspection. 1.2.2 Determination Polymerase chain reaction (PCR, urine samples were 2 500 r / min centrifugation 10 min, to remove cell debris, supernatant was collected. Since PCR inhibitors in the urine, so need to use polyethylene glycol (PEG6000 were Pr