Nested MSP detection of acute leukemia in patients with p16 gene methylation status.doc

 PAGE \* MERGEFORMAT 20 Nested MSP detection of acute leukemia in patients with p16 gene methylation status Author: Fan Liping Shen Jian-zhen Ye Bao-Guo Lin Fuan FU Hai-ying Zhou Huarong Shen Song Fei Yu Aifang 【Abstract】 In order to study p16 gene methylation and deletion and the relationship between the incidence of acute leukemia to explore the occurrence and development of acute leukemia in adults, the biological significance of nested methylation-specific polymerase chain reaction (nested methylation specific polymerase chain reaction, n  MSP) analysis of 82 patients with various subtypes of acute leukemia patients at different stages in newly diagnosed or recurrent p16 gene methylation and deletion status, and sulfide-modified genomic sequencing of PCR and cloned verify the accuracy of the results of . The results showed that: 82 patients with acute leukemia (AL) in patients with p16 gene methylation of the occurrence rate was 39.0%, including acute myeloid leukemia (AML) patients with 41.4%, 24 cases of acute lymphoblastic leukemia (ALL) patients was 33.3%; AL in patients with newly diagnosed 36.6%, while patients with recurrence, compared with 54.5%. 82 cases of AL patients were six cases of p16 gene deletion, loss rate of 7.3% in AML, ALL patients were 1.7% and 20.8%. 16 cases of healthy volunteers or non-malignant hematological disease did not occur in patients with p16 gene methylation or missing. Conclusion: In adults, the occurrence and development of acute leukemia, p16 gene methylation compared with homozygous deletions of p16 gene is more meaningful; p16 gene abnormalities and the occurrence and development of adult acute leukemia are closely related. Keywords: p16 gene; gene methylation; acute leukemia; n  MSP Detection of p16 Gene Methylation Status in Adult Patients with Acute Leukemia by Using n  MSP Abstract The study was aimed to explore the relationship between patterns of methylation or deletion and