Neural stem cell transplantation for treatment of spinal cord injury in rats in vivo tracing study.doc

 PAGE \* MERGEFORMAT 22 Neural stem cell transplantation for treatment of spinal cord injury in rats in vivo tracing study Authors: Li-Feng Zhang, Zu-Bin Mao, Chiang Tsan Lee 【Abstract】 Objective: To explore the use of MR technology to track stem cells in vivo feasibility and neural stem cells (NSCs) transplantation on rats with spinal cord injury (SCI) after functional recovery. Methods: 66 SD rats were randomly divided into sham-injury group (A group), SCI control group (B group), and cell transplantation in the treatment group (C group) 3 groups, the application has been coated with poly-L-lysine (PLL ) and SPIO marks NSCs, pairs of labeled cells to Prussian blue staining, respectively 1,2,3,4,5 weeks after cell transplantation of each group of animals BBB score 1,3,5 weeks after cell transplantation line MRI examination . Results: (a) Prussian blue staining confirmed that the method of NSCs were marked 100% efficiency. (2) cells 1 ~ 5 weeks after transplantation, B, C group animals have different levels of motor function recovery, but the B group to resume a slow, BBB score difference was statistically significant (P lt;0.05). (3) 1.5 T MRI examination, see the migration showed low signal in T2WI sequence changes, the first 3 weeks, when low signal to the damage zone expansion, the first 5 weeks when the damage zone can be seen in the low signal to change. Conclusion: MR technology to track stem cells in vivo, NSCs transplantation for treatment of SCI is conducive to the functional recovery of rat hind limb. Keywords: Spinal cord injury; neural stem cell transplantation; magnetic resonance imaging; rat Spinal cord injury (spinal cord injury, SCI) after very limited capacity for self-repair, the death of neurons can not be self-generated neurons or neighboring neurons instead. In recent years, neural stem cells (neural stem cells, NSCs) research has changed this view. NSCs in the central nervous system has a capacity of proliferation