Neural stem cells combined use of valproic acid into rat spinal cord injury repair.doc

 PAGE \* MERGEFORMAT 14 Neural stem cells combined use of valproic acid into rat spinal cord injury repair Of: Wang Li Lin Wu Yamin Liu Yuan had southern New [Abstract] Objective To investigate the neural stem cells (NSCs) and valproic acid (VPA) in combination in adult rats after spinal cord injury repair. Methods SD rats were randomly divided into simple injury, NSCs transplantation group, and NSCs + VPA treatment group . with spinal cord hemisection injury model, pathological staining after injury and nerve evoked potential. Results NSCs + VPA treated groups injury at the cavity closure was better than the other two groups, graft and host spinal cord fiber link established more than the other two groups, orderly arranged. NSCs + VPA treatment group evoked even better than the other two indicators, the most significant 8 weeks, of which the motor evoked potential (MEP optimization of the most obvious. Conclusions NSCs and the combined use of VPA into rat spinal cord injury good repair. [Keywords:] spinal cord injury in neural stem cells wound healing valproic acid After spinal cord injury because the damaged neurons do not regenerate, but a large number of glial cell proliferation, a serious impediment to the recovery of spinal cord function. Neural stem cells (neural stem cells, NSCs transplanted into spinal cord injury in the current hot spots, but a lot of research reports, differentiation of NSCs transplanted into the body mainly glial cells, it can not repair the injured spinal cord good. the present study, the treatment of epilepsy drug valproic acid used (valproic acid, VPA and NSCs transplants, spinal cord injury is proposed for solving the existing the provision of new ideas. Materials and methods 1 NSCs Culture and identification The body weight is 220 ~ 250g of E13 ~ 15 SD pregnant rats (Daping Hospital, Third Military Medical University Animal Center) 1, 1% sodium pentobarbital (40mg/kg) were anesthetized by intraperitoneal