New Zealand rabbit spinal cord within the water channel protein in.doc

 PAGE \* MERGEFORMAT 16 New Zealand rabbit spinal cord within the water channel protein in Author: Sun Guozhu, ZHANG Qing-jun, Zhao Zongmao, Zhang Geng Shen, Wang Hao [Keywords:] aquaporins Expression and distribution of aquaporin4 in spinal cord of New Zealand rabbits [Abstract] AIM: To examine the expression and distribution of aquaporin4 (AQP4) in normal spinal cord of rabbits, so as to provide theoretical basis for the role of AQP4 in spinal water transport and balance regulation. METHODS: Immunohistochemistry and in situ hybridization were performed on spinal paraffin sections from healthy adult rabbits to detect the expression and distribution of AQP4 protein and mRNA. RESULTS: The positive cells mostly included ependymal cells of myelocoele, glial cells, anterior horn motor neurons, and epithelial cells of spinal pia mater and their expressions were mainly located on cell membrane. Interestingly, AQP4 distribution in glial cells and ependymal cells showed certain directivity, but the former was mainly in the membrane side contacting with capillary endothelial cells and the latter mostly in the basolateral membrane of ependymal cells. CONCLUSION: AQP4 is widely expressed in rabbit spinal cord and its anatomical locations suggest the AQP4 may be involved in spinal cord water transport and balance regulation and electrolyte metabolism, even can be locally regulated to meet special functional request. [Keywords] aquaporin4; rabbitss; spinal cord; immunohistochemistry; in situ hybridization [Abstract] Objective: To evaluate the normal water channel protein in the spinal cord of New Zealand white rabbits 4 (AQP4) position and expression, in order to study the metabolism of water in the spinal cord to provide theoretical basis for the role. Methods: Normal adult New Zealand white rabbits, using immunohistochemical and in situ hybridization to detect spinal cord AQP4 protein and mRNA expression of the location and. Results: Immunohistochemical staining and