NK cells in human nasopharyngeal carcinoma cells in nude mice transplanted tumor CNE2 inhibition of.doc

 PAGE \* MERGEFORMAT 2 NK cells in human nasopharyngeal carcinoma cells in nude mice transplanted tumor CNE2 inhibition of Author: Mei users to switch, Guo Yuan, WU Yuan-Bin, Zhou Jian, WEI Hong-Mei [Abstract] Objective To study the allogeneic NK cells against human nasopharyngeal carcinoma cells (CNE2) subcutaneous tumor in nude mice in vivo. Methods PCR  SSP assay CNE2 cell HLA  A, B, Cw phenotype, NK cell KIR phenotype (3 cases of healthy choices for the test object), NK cells isolated by magnetic bead separation and cultured in vitro amplification, LDH release Determination of NK cells in vitro cytotoxic activity of CNE2 cells. 12 BALB / c nude mice were divided into two groups of six in the control group were inoculated subcutaneously in nude mice each 1 * 106CNE2 cells, the treatment group were inoculated subcutaneously in nude mice each 1 * 106CNE2 cells injected via the tail vein at the same time each 3 * 107NK cells were observed in nude mice tumor formation time and the rate of tumor formation, tumor volume change, calculate inhibitory rate. Results CNE2 cell surface HLA  A, B, Cw and phenotype was A2, 24; B18, 35; Cw4, 7,3 caught healthy expression of KIR2DL1, KIR2DL3, KIR3DL1, KIR3DL2. 5:1,10:1,20:1,30:1 effect when the target ratio, NK cell killing activity of CNE2 cells, respectively (9.37 ± 2.14)%, (27.14 ± 1.82)%, (36.40 ± 4.28)% and (54.67 ± 2.80)%. NK cells in the control group and treatment group, tumors appeared time was (10.00 ± 2.68) d, (18.80 ± 1.64) d, (P